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Studie belegt: Aromenkonzentrationen viel zu niedrig um Bronchialgewebe zu schädigen

Die Forscher Lukasz Czekala, Liam Simms, Matthew Stevenson, Edgar Trelles-Sticken, Paul Walker, Tanvir Walele haben im August 2019 die Studie mit dem Namen: High Content Screening in NHBE cells shows significantly reduced biological activity of flavoured e-liquids, when compared to cigarette smoke condensate herausgebracht.
Interessant ist dabei das außer beim Menthol keine Verhaltensauffälligkeiten festgestellt wurden. Und auch hier müssten die Mengen um 100 Prozent 100-mal höher sein als in handelsüblichen Aromen oder Liquids.

Conclusion

In this study, we used HCS to compare the biological effects of experimental and commercial e-liquids with 3R4F CSC in NHBE cells in a range of endpoints covering cellular health, energy production and oxidative stress. All tested e-liquids (with or without nicotine and flavourings) were typically non-cytotoxic and had no effect on most HCS endpoints at concentrations below 1%. The only e-liquid that impacted cell count (below 1% testing concentration) was CF Menthol, however the MEC value for this endpoint was 360 times higher than for 3R4F CSC. Our results clearly shows a lower toxicity of e-liquids, including flavoured e-liquids, when compared to CSC in this experimental setting. Typically, more than 100 times higher concentrations of CFs are required to elicit the same response as those as those observed for 3R4F CSC in specific endpoints.

While the presence of flavours in CFs triggered some effects (e.g. cell cycle arrest) not seen with base liquid formulation with or without nicotine, these effects were only observed at concentrations several orders of magnitude higher than CSC and at levels above which humans are likely to be exposed to, through daily usage. However, flavours play a critical role in attracting, and retaining smokers to e-cigarettes and are providing a viable alternative to combusted tobacco.

HCS has been shown to be a robust tool to screen e-liquids for mechanistic, multiparametric information on toxicity and presents a valuable tool for stewardship and prioritization purposes, when used as part of an overall Weight of Evidence approach (WoE) with other assays (including in vitro and clinical studies). We have undertaken further studies on e-cigarette aerosols using 3D organotypic models, offering more robust testing platform, to gain a better understanding of in vivo toxicity more closely recapitulating human exposure scenario and this work will be presented in a future publication.

Lukasz Czekala, Liam Simms, Matthew Stevenson, Edgar Trelles-Sticken, Paul Walker, Tanvir Walele

Erklärung der Abkürzungen:

• HCS= High Content Screening
• CSC= Cigarette Smoke Condensate
• NHBE = Normal Human Bronchial Epithelial
• CF= commercial, flavoured, nicotine-containing e-liquid